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Post by Dominik Buschmann on May 29, 2018 19:41:23 GMT 2
Someone has to start with the introductions, so I'll just go ahead and post the first thread!
Name: Dominik Buschmann
Location: Freising, Germany
University: Technical University of Munich (TUM)
Department: Animal Physiology and Immunology
Status: PhD Student
Research interests:
- Circulating biomarkers in critically ill patients
- Role of EVs and circulating miRNAs in sepsis
- EV isolation and characterization
- EV transcriptomics (small RNA, miRNAs, isomiRs, lncRNAs)
- Milk EVs
Tools:
- Various methods for isolation of EVs from biofluids (kit-based and others)
- EV characterization: WB, NTA, TEM
- Next-Gen Sequencing
- RT-qPCR
Looking forward to many great discussions on this platform!
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Post by Fabia Fricke on May 30, 2018 8:26:39 GMT 2
Name: Fabia Fricke
Location: Heidelberg, Germany
University: University of Heidelberg, Medical Faculty
Institute: German Cancer Research Center (DKFZ), Institute of Pathology at University Hospital Heidelberg
Department: Applied Tumor Biology
PIs: Prof. Dr. Jürgen Kopitz, Dr. Johannes Gebert
Status: PhD Student
Research interests: "TGFBR2-dependent priming of MSI colorectal tumor cell-derived exosomes – proteomic, mutagenomic, diagnostic profiling" i) Technical relevance: Immunoaffinity-based enrichment strategies for isolation of cell type-specific EVs ii) Biological relevance: Biology and function of EVs in microsatellite unstable colorectal carcinomas iii) Clinical relevance: Minimal-invasive MSI typing using tumor-derived EVs
Tools: - EV isolation: various methods (including ultracentrifugation, PEG-based isolation, SEC); from cell culture medium, patient samples - EV characterization: TEM, NTA, WB - PCR-based frameshift analysis - LC-MS/MS Proteomics, Phosphoproteomics - Multiplex Analysis of the Secretome (Luminex technology) - Metabolic-labeling strategies using radioisotopes
Comment: Thank you very much for the establishment of such a great online forum! I am really looking forward to numerous of interesting discussions and scientific exchange. Especially, I would highly like to encourage the "Young Investigators" and "Young EV Researchers" to get in touch. It will be beneficial for all of us, if we use this forum to communicate and to exchange our experiences. Lets get started!
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Post by chpreusser on May 30, 2018 10:06:41 GMT 2
Name: Christian Preußer
Location: Gießen, Germany University: Justus Liebig University Gießen,
Institute: Institute of Biochemistry
PI: Prof. Dr. Albrecht Bindereif
Status: Postdoc
Research interests:
EV isolation and characterization circular RNAs in EVs (unraveling the potential as a new class of biomarkers) EV transcriptomics (circRNAs, mRNAs, ncRNAs) EVs in parasitic infections (T. brucei, P. falciparum, etc.)
Tools:
- RNA biochemistry: (RT-qPCR, Northern Blot, affinity purification of RNA-protein (various assays), in vitro transcription, etc.)
- RNomics (Next-Gen-sequencing, RNA-protein interactions: Cross-linking and immunoprecipitation, in combination with deep-sequencing (iCLIP-Seq), Fluorescence in situ hybridization (RNA)
- EV isolation: various methods (including ultracentrifugation, SEC) from cell culture medium, blood samples
- EV characterization: NTA, WB
Thanks for the setup of this great forum. Looking forward for interesting discussions!
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Dr Jagan Billakanti
Guest
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Post by Dr Jagan Billakanti on Sept 10, 2021 8:29:03 GMT 2
Hi everyone, I am Dr Jagan Billakanti working as a Downstream Process Development and purification of bioactive molecules for Cytiva and based in Australia. For the past 16 years, i have been in this field helping customer for purification proteins, recombinant proteins, antibodies, VLPS, viruses, EVs and many more molecules at lab to production scale. My interest in this group to understand the bottle-necks of EVs purification and help the community where appropriate and also accelerate the commercialisation of EVs therapeutics by providing a scalable downstream strategy. Thank you. Kind regards, Jagan My email is jagab.billakanti@cytiva.com or billakantij@gmail.com.
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