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Post by Fabia Fricke on May 30, 2018 8:36:44 GMT 2
Dear young EV scientists and junior researchers,
we would really like to get in touch with you to support and promote your EV research and career at an early stage of development. To get in contact, we would like to encourage you to answer the following questions:
1. What is your name?
2. In which institute/department are you working?
3. What is your position/stage of professional education? (Are you a bachelor-, master or PhD student?)
4. Can you remember your first EV isolation or experiment? (When was it? How was it going?)
5. What is the focus of your EV research?
6. How can we support your personal and professional development? What kind of support would you like to have from the GSEV?
(Optional: Do you have any other comments, wishes or requests?)
We are looking forward to get in touch!
Best regards, Dominik and Fabia
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Post by Fabia Fricke on May 30, 2018 9:47:19 GMT 2
Here is my short introduction 1. What is your name? Fabia Fricke 2. In which institute/department are you working? Applied Tumor Biology, Institute of Pathology, University Hospital Heidelberg, German Cancer Research Center (DKFZ) 3. What is your position/stage of professional education? PhD Student since autumn 2016 4. Can you remember your first EV isolation or experiment? The first time I have isolated EVs was in 2015. We aimed to isolate nanovesicles from cell culture supernatant. I really enjoyed the lab work including differential centrifugation, ultracentrifugation and also PEG-based precipitation. At the end, I have visualised the isolated EVs using TEM. It was amazing to see such small vesicles. Since this first isolation, we have worked a lot on the optimisation of our running protocols. The EV lab work and characterisation are challenging and exciting! 5. What is the focus of your EV research? TGFBR2-dependent priming of MSI colorectal tumor cell-derived exosomes – proteomic, mutagenomic, diagnostic profiling 6. How can we support your personal and professional development? What kind of support would you like to have from the GSEV? First, I would like to encourage many researchers to participate in our community. The more people we are, the better we can interact with each other and share our experiences. Second, together we can overcome technical and theoretical challenges, if we cooperate and communicate across laboratories and working groups sharing our know-how and expertise. Third, GSEV will support us to be "up-to-date" in the improvement of EV isolation, characterisation and other EV-related experimental designs. In this way, we ensure that most of us are aware of the current limitations/challenges within the EV field, but also enthusiastic to work on the potential high future impact of EVs. Do you have any other comments, wishes or requests? We as young scientists, have a great importance for the future of this exciting research field. Lets try our best, to work thoughtful and concentrated on the current challenges in a collaborative-manner to contribute as best as possible to the basic concepts of EV science. Therefore, lets discuss our ideas and experiences!
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Alexandre Felipe S. A. de Lima
Guest
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Post by Alexandre Felipe S. A. de Lima on Nov 9, 2023 6:08:51 GMT 2
1. What is your name? Alexandre (Alex) 2. In which institute/department are you working? Chinese Academy of Sciences (Beijing, China) - Laboratory of Protein and Peptide Pharmaceuticals & Proteomics Laboratory - Institute of Biophysics 3. What is your position/stage of professional education? (Are you a bachelor-, master or PhD student?) Masters student 4. Can you remember your first EV isolation or experiment? (When was it? How was it going?) Isolation of EV from human plasma using asymmetrical flow field flow fractionation (AF4). Working on protocol improvement. 5. What is the focus of your EV research? Use of Electrical Asymmetrical Field Flow Fractionation for isolation of exosomes from human plasma for use in peptidomics analysis 6. How can we support your personal and professional development? What kind of support would you like to have from the GSEV? I am current doing my master in China working with EV. I will move to Berlin in 2024 to do my PhD after finishing my master. Thus, I am looking to establish connections with Berlin laboratories and other cities working with EV. My LinkedIn is: / Looking forward to hearing from you.
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